Journal: STAR Protocols

Article Title: Protocol for an open-source system to integrate calcium imaging, pupillometry, and locomotion-estimated tracking in head-fixed mice

doi: 10.1016/j.xpro.2024.103331

Figure Lengend Snippet:

Article Snippet: Spinnaker SDK USB3 Vision Cameras Driver , Teledyne FLIR , https://www.flir.com/products/spinnaker-sdk/.

Techniques: Recombinant, Ointment, Cream, Saline, Software, Imaging, Polymer, Stripping Membranes, Blocking Assay, Battery, Adhesive, Microscopy

Journal: Frontiers in Immunology

Article Title: Maternal antibiotic exposure-mediated alterations in basal, and allergen-induced lung function are associated with altered recruitment of eosinophils to the developing lung

doi: 10.3389/fimmu.2025.1715675

Figure Lengend Snippet: Maternal ABX exposure induces dysbiosis in offspring and is associated with reduced SCFA production. Nursing dams were given access to water supplemented with 0.5 mg/ml sucralose ± 1mg/ml each Ampicillin, Vancomycin, Gentamicin (red squares), or sucralose alone (black circles) from PN10 to PN20. Fecal samples were collected longitudinally from offspring at the indicated times and subjected to metagenomic analysis to identification of bacterial Families. n = 5 mice/group at each time point (A) . Fecal pellets were collected at PN28, and levels of (B) Acetate, (C) Butyrate, and (D) Propionate were measured by NMR. Mean ± SEM shown. As a Kolmogorov-Smirnov test demonstrated these data did not follow a normal distribution, a Mann-Whitney test was used. ** and **** indicate p<0.01 and p<0.0001 between Sucralose and ABX-exposed animals. n = 17–21 mice/group from 3 experiments.

Article Snippet: SCFA assignment and quantifications were performed using Chenomx ® NMR Suite profiling software (Chenomx Inc. version 8.4) based on the TMSP (0.33 mM).

Techniques: MANN-WHITNEY

Journal: eLife

Article Title: Traumatic injury compromises nucleocytoplasmic transport and leads to TDP-43 pathology

doi: 10.7554/eLife.67587

Figure Lengend Snippet:

Article Snippet: Immunostaining was visualized using a 3,3'-diaminobenzidine (DAB) staining kits (Vector Laboratories, #SK-4100) for ~5 min.

Techniques: CRISPR, Transfection, Construct, Plasmid Preparation, Transduction, Sequencing, Enzyme-linked Immunosorbent Assay, Software